rxYFP

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GFP from Aequorea victoria , wild-type form.

rxYFP ( English Redox-sensitive YFP ) is a modified variant of the green fluorescent protein , in which the fluorescence changes depending on the redox .

properties

In comparison to the wild-type form of GFP, rxYFP has two oxidizable cysteines . By oxidation of the thiol groups is of the cysteines cystine formed, which affects in a reduction of the fluorescence of the rxYFP.

Applications

The rxYFP is used, among other things, as a redox-dependent biosensor and as a reporter protein. With rxYFP, the redox potential and the amount of glutathione in the cytosol or in the cell nucleus can be determined in vivo .

Alternative optical detection methods use e.g. B. Resazurin (synonym AlamarBlue), dichlorofluorescein (DCF) or the proteins HyPer or roGFP .

Individual evidence

  1. ↑ H. Østergaard, C. Tachibana, JR Winther: Monitoring disulfide bond formation in the eukaryotic cytosol. In: The Journal of Cell Biology . Volume 166, Number 3, August 2004, pp. 337-345, ISSN  0021-9525 . doi : 10.1083 / jcb.200402120 . PMID 15277542 . PMC 2172265 (free full text).
  2. ↑ KA Lukyanov, VV Belousov: Genetically encoded fluorescent redox sensors. In: Biochimica et Biophysica Acta . Volume 1840, Number 2, February 2014, pp. 745-756, ISSN  0006-3002 . doi : 10.1016 / j.bbagen.2013.05.030 (free full text). PMID 23726987 .
  3. ↑ M. Dardalhon, C. Kumar, I. Iraqui, L. Vernis, G. Kienda, A. Banach-Latapy, T. He, R. Chanet, G. Faye, CE Outten, ME Huang: Redox-sensitive YFP sensors monitor dynamic nuclear and cytosolic glutathione redox changes. In: Free radical biology & medicine. Volume 52, Numbers 11-12, 2012 Jun 1-15, pp. 2254-2265, ISSN  1873-4596 . doi : 10.1016 / j.freeradbiomed.2012.04.004 . PMID 22561702 . PMC 3382975 (free full text).
  4. ↑ A. Banach-Latapy, T. He, M. Dardalhon, L. Vernis, R. Chanet, ME Huang: Redox-sensitive YFP sensors for monitoring dynamic compartment-specific glutathione redox state. In: Free Radical Biology and Medicine . Volume 65, December 2013, pp. 436-445, ISSN  1873-4596 . doi : 10.1016 / j.freeradbiomed.2013.07.033 . PMID 23891676 .
  5. ↑ S. Pouvreau: Genetically encoded reactive oxygen species (ROS) and redox indicators. In: Biotechnology journal. Volume 9, Number 2, February 2014, pp. 282-293, ISSN  1860-7314 . doi : 10.1002 / biot.201300199 . PMID 24497389 .