Vero cells

In Vero cells is an established continuous cell line derived from normal kidney cells of African green monkeys was recovered. "Vero" itself is the abbreviation of two Esperanto words ("verda" and "reno"), which means "green kidney" when translated.

They have a fibroblast-like morphology and are typically cultured in DMEM ( Dulbecco's Modified Eagle Medium ) + 2 mM glutamine + 10% fetal calf serum .

Vero cells can be infected with a number of viruses , e.g. B. Influenza , polio , rubella , alpha , reo or rabies viruses .

Examples

A Vero E6 cell (blue) that was infected with SARS-CoV-2 . Colored image of a scanning electron microscope .

The following subcell lines or mutants are mentioned as examples:

Vero 317: cells derived from Vero 303. They grow in a glutamine-free medium without FCS. Their morphology is more similar to epithelial cells.
Vero C1008: Show slight contact inhibition and are suitable for slowly replicating viruses. similar to epithelial cells
Vero / hSLAM: have a surface receptor (hSLAM or CDw150) that is required for the measles virus to take up into the cell. fibroblast-like morphology

use

Vero cells are used in the vaccine production of rotaviruses ( e.g. Rotarix or RotaTeq ), some inactivated polio vaccines ( e.g. Imovax Polio or Hexyon ) and the inactivated Japanese encephalitis vaccine Ixiaro . However, Vero cells are not an ingredient in these or any other vaccines . They are only used to breed the respective viruses and are therefore not part of vaccinations .

Web links

Individual evidence

^ Stanley A. Plotkin et al .: Plotkin's Vaccines . 7th edition. Elsevier, 2017, ISBN 978-0-323-35761-6 , pp. 928 ( elsevier.com ).
↑ A. Sabchareon et al .: A new Vero cell rabies vaccine: results of a comparative trial with human diploid cell rabies vaccine in children . In: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America . tape 29 , no. 1 , July 1999, p. 141-149 , doi : 10.1086 / 520143 , PMID 10433577 .
↑ National Institute of Allergy and Infectious Diseases : Novel Coronavirus SARS-CoV-2 . In: flickr .com . March 20, 2020, Retrieved March 22, 2020.
↑ Specialist information Rotarix. (PDF) In: EMA . April 2019, accessed March 22, 2020 .
↑ Specialist information RotaTeq. (PDF) In: EMA. December 2018, accessed March 22, 2020 .
↑ technical information Imovax polio. (PDF) In: Dimdi . September 2015, accessed March 22, 2020 .
↑ technical information Hexyon. (PDF) In: EMA. June 2019, accessed March 22, 2020 .
^ Specialist information Ixiaro. (PDF) In: EMA. January 2020, accessed on March 22, 2020 .
↑ Joana Splieth: No, cells from human fetuses and monkeys or glyphosate are not ingredients of vaccinations. In: corrective . December 13, 2019, accessed March 22, 2020 .

[1] Stanley A. Plotkin et al .: Plotkin's Vaccines . 7th edition. Elsevier, 2017, ISBN 978-0-323-35761-6 , pp. 928 ( elsevier.com ).

[2] A. Sabchareon et al .: A new Vero cell rabies vaccine: results of a comparative trial with human diploid cell rabies vaccine in children . In: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America . tape 29 , no. 1 , July 1999, p. 141-149 , doi : 10.1086 / 520143 , PMID 10433577 .

[3] National Institute of Allergy and Infectious Diseases : Novel Coronavirus SARS-CoV-2 . In: flickr .com . March 20, 2020, Retrieved March 22, 2020.

[4] Specialist information Rotarix. (PDF) In: EMA . April 2019, accessed March 22, 2020 .

[5] Specialist information RotaTeq. (PDF) In: EMA. December 2018, accessed March 22, 2020 .

[6] technical information Imovax polio. (PDF) In: Dimdi . September 2015, accessed March 22, 2020 .

[7] technical information Hexyon. (PDF) In: EMA. June 2019, accessed March 22, 2020 .

[8] Specialist information Ixiaro. (PDF) In: EMA. January 2020, accessed on March 22, 2020 .

[9] Joana Splieth: No, cells from human fetuses and monkeys or glyphosate are not ingredients of vaccinations. In: corrective . December 13, 2019, accessed March 22, 2020 .