Weibel Palade corpuscles
| Parent |
| Organelle |
| Gene Ontology |
|---|
| QuickGO |
Weibel-Palade-Bodies (WPK) are approximately 0.1 × 2 µm in size cell organelles that are predominantly located close to the nucleus in the cytoplasm of the cells of the inner walls of blood vessels . In their internal tubular structure, they store the Von Willebrand factor , which promotes blood clotting , interleukin-8 , which attracts white blood cells , the transmembrane protein P-selectin , which binds it, and angiopoietin- 2, which mobilizes stem cells . Physiological agonists such as thrombin , histamine or uric acid , which arise when tissues are insufficiently supplied with oxygen ( ischemia ), can induce the release of these substances into the direct cell environment within seconds. If inflammatory reactions are present, the number of Weibel-Palade bodies almost doubles.
In the context of a direct representation of the cell organelles with the aid of a transmission electron microscope , mostly only a few small sections of the Weibel-Palade corpuscles can be seen. Via immunofluorescence these can be characteristic in shape and size the vesicles represent but easily when due to their intense expression of factor VIII - antigen with highly specific fluorescent antibodies are marked.
Web links
- Electron microscope image of Weibel-Palade bodies in cross section
- Electron microscope image of Weibel-Palade bodies in longitudinal section ( Memento from June 7, 2007 in the Internet Archive )
literature
- ER Weibel, GE Palade: New cytoplasmic components in arterial endothelia . In: J. Cell Biol . October 23, 1964, pp. 101-12. doi : 10.1083 / jcb.23.1.101 . PMID 14228505 . PMC 2106503 (free full text).
Individual evidence
- ↑ Mei-Chuan Kuo, Daniel Patschan, Susann Patschan, Leona Cohen-Gould, Hyeong-Cheon Park, Jei Ni, Francesco Addabbo, Michael S Goligorsky: Ischemia-induced exocytosis of Weibel-Palade bodies mobilizes stem cells . In: Journal of the American Society of Nephrology . 19, No. 12, December 2008, pp. 2321-2330. doi : 10.1681 / ASN.2007111200 . PMID 18715993 .