3,3'-diaminobenzidine

Structural formula
General
Surname	3,3'-diaminobenzidine
other names	2,2 ', 4,4'-biphenyltetramine 3,3 ', 4,4'-tetraaminobiphenyl
Molecular formula	C 12 H 14 N 4
Brief description	brown odorless solid
External identifiers / databases
CAS number 91-95-2 EC number 202-110-6 ECHA InfoCard 100.001.919 PubChem 7071 ChemSpider 6804 Wikidata Q4030650
properties
Molar mass	214.27 g mol −3
Physical state	firmly
Melting point	175-177 ° C
solubility	little in water (0.55 g l −1 at 20 ° C)
safety instructions
GHS hazard labeling from  Regulation (EC) No. 1272/2008 (CLP) , expanded if necessary danger H and P phrases H: 341-350 P: 201-308 + 313
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

3,3′-Diaminobenzidine (DAB) belongs to the group of benzidines .

properties

3,3'-Diaminobenzidine is used in biochemistry as a chromogenic substrate for immunostaining with a horseradish peroxidase and, after oxidation with hydrogen peroxide, provides a brown dye that is insoluble in water but soluble in ethanol , e.g. B. in an immunoperoxidase assay , western blot and immunohistochemistry .

literature

• T. Mitsui: Development of peroxidase histochemistry. In: The Tokai journal of experimental and clinical medicine. Volume 9, Number 3, August 1984, pp. 167-173, PMID 6100404 .

Individual evidence

1. ↑ ^{a b c d e} Entry on 3,3′-diaminobenzidine in the GESTIS substance database of the IFA , accessed on January 8, 2018(JavaScript required) .
2. ↑ Entry on biphenyl-3,3 ′, 4,4′-tetrayltetraamine in the Classification and Labeling Inventory of the European Chemicals Agency (ECHA), accessed on August 1, 2016. Manufacturers or distributors can expand the harmonized classification and labeling .
3. ^ JA Litwin: Histochemistry and cytochemistry of 3,3'-diaminobenzidine. A review. In: Folia histochemica et cytochemica. Volume 17, Number 1, 1979, pp. 3-28, PMID 220157 .