3,3'-diaminobenzidine
Structural formula | |||||||||||||||||||
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General | |||||||||||||||||||
Surname | 3,3'-diaminobenzidine | ||||||||||||||||||
other names |
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Molecular formula | C 12 H 14 N 4 | ||||||||||||||||||
Brief description |
brown odorless solid |
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properties | |||||||||||||||||||
Molar mass | 214.27 g mol −3 | ||||||||||||||||||
Physical state |
firmly |
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Melting point |
175-177 ° C |
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solubility |
little in water (0.55 g l −1 at 20 ° C) |
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safety instructions | |||||||||||||||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
3,3′-Diaminobenzidine (DAB) belongs to the group of benzidines .
properties
3,3'-Diaminobenzidine is used in biochemistry as a chromogenic substrate for immunostaining with a horseradish peroxidase and, after oxidation with hydrogen peroxide, provides a brown dye that is insoluble in water but soluble in ethanol , e.g. B. in an immunoperoxidase assay , western blot and immunohistochemistry .
literature
- T. Mitsui: Development of peroxidase histochemistry. In: The Tokai journal of experimental and clinical medicine. Volume 9, Number 3, August 1984, pp. 167-173, PMID 6100404 .
Individual evidence
- ↑ a b c d e Entry on 3,3′-diaminobenzidine in the GESTIS substance database of the IFA , accessed on January 8, 2018(JavaScript required) .
- ↑ Entry on biphenyl-3,3 ′, 4,4′-tetrayltetraamine in the Classification and Labeling Inventory of the European Chemicals Agency (ECHA), accessed on August 1, 2016. Manufacturers or distributors can expand the harmonized classification and labeling .
- ^ JA Litwin: Histochemistry and cytochemistry of 3,3'-diaminobenzidine. A review. In: Folia histochemica et cytochemica. Volume 17, Number 1, 1979, pp. 3-28, PMID 220157 .