4-chloro-1-naphthol

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Structural formula
Structural formula of 4-chloro-1-naphthol
General
Surname 4-chloro-1-naphthol
Molecular formula C 10 H 7 ClO
External identifiers / databases
CAS number 604-44-4
EC number 210-068-5
ECHA InfoCard 100.009.153
PubChem 11787
Wikidata Q22668708
properties
Molar mass 178.61 g mol −1
Physical state

firmly

Melting point

118-121 ° C

safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

4-chloro-1-naphthol is a chemical compound that belongs to the group of naphthols .

properties

4-chloro-1-naphthol is used in biochemistry as a chromogenic substrate for immunostaining with a horseradish peroxidase and, after oxidation with hydrogen peroxide in the presence of dimethyl or diethyl analogues of p - phenylenediamine or o -phenylenediamine, produces via the Nadi reaction a blue dye or, in the presence of 3-methyl-2-benzothiazolinonhydrazone, a red dye, e.g. B. ELISA , Western blot and immunohistochemistry .

Individual evidence

  1. ↑ a b c data sheet 4-chloro-1-naphthol from Sigma-Aldrich , accessed on February 20, 2018 ( PDF ).
  2. ↑ SM Conyers, DA Kidwell: Chromogenic substrates for horseradish peroxidase. In: Analytical biochemistry. Volume 192, Number 1, January 1991, pp. 207-211, PMID 2048722 .
  3. ^ J. Uhl, RC Newton: Quantitation of related proteins by Western blot analysis. In: Journal of immunological methods. Volume 110, Number 1, May 1988, pp. 79-84, PMID 3286776 .
  4. ↑ R. Kobayashi, Y. Tashima: Visualization of antigen on nitrocellulose membrane by the oxidative coupling reaction of N, N′-dimethyl-p-phenylenediamine and 4-chloro-1-naphthol. In: Analytical biochemistry. Volume 183, Number 1, November 1989, pp. 9-12, PMID 2482680 .