Adenovirus early region 1A protein

Early E1A protein
other names	Early E1A 32 kDa protein
	Existing structural data : PDB 2KJE , PDB 2R7G
Mass / length primary structure	289 amino acids , 31,851 Da
Identifier
External IDs	UniProt : P03255 ;
	Orthologue

Adenovirus early region E1A protein (E1A) is a protein from the human adenovirus . It is in biochemistry and cell biology for the immortalization of cells used in mice, hamsters and rats.

properties

The adenovirus early region E1A protein is used by the adenovirus to replicate the viral genome . Furthermore, transforms it host cells from mice, hamsters and rats. However, only E1A proteins from some serotypes (AdV12) are oncogenic . while the transformed cells of other non-oncogenic serotypes (AdV2 and AdV5) are lysed in vivo by NK cells . It brings dormant cells to cell division by overcoming the checkpoints of the cell from the G ₀ phase to the S phase . In the cell, it binds to pRB (synonymous RB1), p300 and CtBp . In addition, it leads to a dissociation of E2F1 from pRB, whereby genes of the S phase and the E2 region of the adenoviruses are expressed . It binds divalent zinc ions and is phosphorylated .

Web links

MeSH adenovirus early region 1A protein

Individual evidence

^ Y. Katakura, S. Alam, S. Shirahata: Immortalization by gene transfection. In: Methods in cell biology. Volume 57, 1998, pp. 69-91, ISSN 0091-679X . PMID 9648100 .
↑ ^a ^b ^c H. Guan, RP Ricciardi: Transformation by E1A oncoprotein involves ubiquitin-mediated proteolysis of the neuronal and tumor repressor REST in the nucleus. In: Journal of virology. Volume 86, number 10, May 2012, pp. 5594-5602, doi : 10.1128 / JVI.06811-11 , PMID 22419809 , PMC 3347283 (free full text).
↑ T. Jelinek, DS Pereira, FL Graham: Tumorigenicity of adenovirus-transformed rodent cells is influenced by at least two regions of adenovirus type 12 early region 1A. In: Journal of virology. Volume 68, Number 2, February 1994, pp. 888-896, PMID 8289391 , PMC 236525 (free full text).
↑ ^a ^b Early E1A protein - Human adenovirus C serotype 5 (HAdV-5). In: uniprot.org. June 20, 2018, accessed May 4, 2018 .

[Katakura-1] Y. Katakura, S. Alam, S. Shirahata: Immortalization by gene transfection. In: Methods in cell biology. Volume 57, 1998, pp. 69-91, ISSN 0091-679X . PMID 9648100 .

[Guan-2] H. Guan, RP Ricciardi: Transformation by E1A oncoprotein involves ubiquitin-mediated proteolysis of the neuronal and tumor repressor REST in the nucleus. In: Journal of virology. Volume 86, number 10, May 2012, pp. 5594-5602, doi : 10.1128 / JVI.06811-11 , PMID 22419809 , PMC 3347283 (free full text).

[3] T. Jelinek, DS Pereira, FL Graham: Tumorigenicity of adenovirus-transformed rodent cells is influenced by at least two regions of adenovirus type 12 early region 1A. In: Journal of virology. Volume 68, Number 2, February 1994, pp. 888-896, PMID 8289391 , PMC 236525 (free full text).

[uniprot-03255-4] Early E1A protein - Human adenovirus C serotype 5 (HAdV-5). In: uniprot.org. June 20, 2018, accessed May 4, 2018 .