Fluorescamine

Structural formula
General
Surname	Fluorescamine
other names	4-Phenylspiro- [furan-2 (3 H ), 1-phthalan] -3,3′-dione ( IUPAC )
Molecular formula	C 17 H 10 O 4
Brief description	White dust
External identifiers / databases
CAS number 38183-12-9 EC number 253-814-5 ECHA InfoCard 100.048.904 PubChem 37927 Wikidata Q238913
properties
Molar mass	278.26 g mol −1
Physical state	firmly
Melting point	153-157 ° C 154-155 ° C
safety instructions
GHS labeling of hazardous substances no GHS pictograms H and P phrases H: no H-phrases P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Fluorescamine is an organic spiro compound that is used in protein analysis .

Fluorescamin is used for fluorescent labeling of amino acids , peptides , sulfonamides and proteins in the picomole range for chromatography and gel electrophoresis . A "free" amino group is derivatized with the reagent:

Fluorescamine shows no fluorescence without derivatisation, the derivative can be excited at 390 nm and then emits at 475 nm. The fluorescence optimum is at pH 9 and fluorescamine is not stable in aqueous solution, so the method is not widely used.

Individual evidence

1. ↑ ^{a b c d} Data sheet Fluorescamine at Sigma-Aldrich , accessed on April 12, 2017 ( PDF ).
2. ↑ European Pharmacopoeia , Deutscher Apotheker Verlag Stuttgart, 6th edition, 2008, p. 605, ISBN 978-3-7692-3962-1 .
3. ↑ Entry on fluorescamine. In: Römpp Online . Georg Thieme Verlag, accessed on April 12, 2017.
4. ↑ Monika Jansohn, Sophie Rothhämel (eds.): Genetic methods: a collection of work instructions for the molecular biological laboratory . 5th edition. Spectrum, Akad. Verl., Heidelberg 2012, ISBN 978-3-8274-2429-7 , pp. 66 .
5. ^ Friedrich Lottspeich, Joachim W. Engels (Ed.): Bioanalytik . 3. Edition. Springer Spectrum, Berlin / Heidelberg 2012, ISBN 978-3-8274-2942-1 , p. 339 f .