Folin's reagent
Structural formula | ||||||||||||||||
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General | ||||||||||||||||
Surname | Folin's reagent | |||||||||||||||
other names |
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Molecular formula | C 10 H 5 NaO 5 S | |||||||||||||||
Brief description |
orange solid |
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properties | ||||||||||||||||
Molar mass | 260.20 g mol −1 | |||||||||||||||
Physical state |
firmly |
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Melting point |
289 ° C |
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solubility |
soluble in water (50 g l −1 at 20 ° C) |
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safety instructions | ||||||||||||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
The Folin reagent , synonymous with sodium 1,2-naphthoquinone-4-sulfonate, is a detection reagent for amines including amino acids from the group of naphthoquinones . Several detection reagents are known under this name, for example an aqueous solution with ammonium sulfate , uranyl acetate and acetic acid for uric acid detection. The Folin reagent is also not identical to the Folin-Ciocalteu reagent and the Folin-Denis reagent . It was named after the Swedish-American biochemist Otto Knut Olof Folin (1867–1934).
properties
In an alkaline environment, the Folin reagent reacts with amines to form a fluorescent dye. Sodium 1,2-naphthoquinone-4-sulfonate is orange and water-soluble at 50 g / l.
Applications
The Folin reagent is used for the photometric determination of amino acids . It is also used to distinguish MDMA from other amphetamines .
substance | Color change |
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MDMA | pink |
MDE | No reaction |
MDA | yellow |
BZP | Orange red |
TFMPP | Pink |
Methylone | No reaction |
Methamphetamine | pink |
amphetamine | Light orange |
PMA | Light yellow |
PMMA | Light orange |
Ketamine | No reaction |
mescaline | No reaction |
cocaine | No reaction |
ASS | No reaction |
Cane sugar | No reaction |
Individual evidence
- ↑ a b c d e f g h data sheet Sodium 1,2-naphthoquinone-4-sulfonate, for spectrophotometric det. of isonicotic hydrazide, amines and amino acids, ≥97.0% (T) from Sigma-Aldrich , accessed on February 15, 2017 ( PDF ).
- ↑ Entry on Folin reagent. In: Römpp Online . Georg Thieme Verlag, accessed on February 16, 2017.
- ↑ Elsevier's Dictionary of Chemoetymology: The Whys and Whences of Chemical Nomenclature and Terminology . Elsevier, 2006, ISBN 978-0-08-048881-3 , pp. 150 ( books.google.de ).
- ↑ Kobayashi Y, Kubo H, Kinoshita T: Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography . In: Anal. Biochem. . 160, No. 2, 1987, pp. 392-8. doi : 10.1016 / 0003-2697 (87) 90066-2 . PMID 3578768 .
- ↑ a b Folin Reagent Testing Kit . Dance Safe. June 2015. Retrieved August 8, 2015.