Jerker Porath

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Jerker Porath (born October 23, 1921 in Sala ; † January 21, 2016 ) was a Swedish biochemist and inventor of methods such as ion exchange chromatography , affinity chromatography and gel permeation chromatography .

biography

Porath studied at Uppsala University and originally did research in organic chemistry under Arne Fredga , where he obtained his licentiate degree. After a scholarship in Heidelberg , he switched to Arne Tiselius' group . Tiselius recommended Porath research stays with Choh Hao Li and in his Hormone Research Laboratory at the University of California, Berkeley , where Porath stayed from 1951 to 1952. He then developed methods of zone electrophoresis and ion exchange chromatography in Uppsala for the purification of hormones . He received his doctorate in 1957 in Uppsala with the dissertation Zone electrophoresis in columns and adsorption chromatography on ionic cellulose derivatives as methods for peptide and protein fractionations: application to the study of posterior pituitary hormones .

Probably his best-known invention is the gel permeation chromatography developed together with Per Flodin , as well as the stationary phases Sephadex and Sepharose . In 1957 Porath discovered the molecular sieve effect of the Sephadex, whereby molecules can be separated according to their size in gel permeation chromatography or their molar mass can be derived from a comparison with the chromatographic running behavior of a comigration standard . Furthermore, Sephadex (cross-linked dextran ) is used for immunoprecipitation and as a stationary phase in affinity chromatography.

From 1969 to 1988 he was a professor at Uppsala University. In 1971 he became a member of the Royal Swedish Academy of Engineering and then the Royal Swedish Academy of Sciences . In 1982 he was accepted as a foreign member of the then Academy of Sciences of the USSR .

Individual evidence

  1. ↑ Forskare som ny väg banade
  2. ↑ a b 80th Birthday of Professor Jerker Porath , in Chromatographia (2001), 54, pp. 431-432. doi : 10.1007 / BF02491195 .
  3. ↑ J. Porath, P. Flodin: Gel filtration: a method for desalting and group separation . In: Nature (1959), Vol. 183 (4676), pp. 1657-9. PMID 13666849 .
  4. ↑ J. Porath: Gel filtration of proteins, peptides and amino acids . In: Biochim Biophys Acta (1960), Vol. 39, pp. 193-207. PMID 14434211 .
  5. ^ P. Andrews: Estimation of the molecular weights of proteins by Sephadex gel filtration. In: Biochem J. (1964), Vol. 91 (2), pp. 222-33. PMID 4158310 ; PMC 1202876 (free full text).
  6. ^ J. Porath: From gel filtration to adsorptive size exclusion . In: J Protein Chem. (1997), Vol. 16 (5), pp. 463-8. PMID 9246630 .
  7. ↑ J. Porath, EB Lindner: Separation methods based on molecular sieving and ion exclusion . In: Nature (1961), Vol. 191, pp. 69-70. PMID 13737223 .
  8. ^ A. Tiselius, J. Porath, PA Albertsson: Separation and fractionation of macromolecules and particles . In: Science (1963) 141 : 13-20 (3575). PMID 13985156 .
  9. ^ R. Axén, J. Porath: Chemical coupling of enzymes to cross-linked dextran ('Sephadex') . In: Nature (1966), Vol. 210 (5034), pp. 367-9. PMID 5963228 .
  10. ^ R. Axén, J. Porath, S. Ernback: Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides . In: Nature (1967), Vol. 214 (5095), pp. 1302-4. PMID 6056841 .
  11. ↑ J. Porath, R. Axén: Immobilization of enzymes to agar, agarose, and Sephadex supports . In: Methods Enzymol. (1976) Vol. 44, pp. 19-45. PMID 1021680 .
  12. ^ Foreign members of the Russian Academy of Sciences since 1724. Jerker Olof Porath. Russian Academy of Sciences, accessed October 16, 2015 (Russian).
personal data
SURNAME Porath, jerker
BRIEF DESCRIPTION Swedish biochemist and inventor
DATE OF BIRTH October 23, 1921
PLACE OF BIRTH Sala (Sweden)
DATE OF DEATH January 21, 2016