Naphthol-AS-MX-Phosphate

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Structural formula
Structure of Naphthol-AS-MX-Phosphate
General
Surname Naphthol-AS-MX-Phosphate
Molecular formula C 19 H 18 NO 5 P
Brief description

light yellow powder

External identifiers / databases
CAS number 1596-56-1
EC number 216-480-1
ECHA InfoCard 100.014.983
PubChem 74136
Wikidata Q22668731
properties
Molar mass 371.32 g mol −1
Physical state

firmly

safety instructions
GHS labeling of hazardous substances
07 - Warning

Caution

H and P phrases H: 315-319-335
P: 261-305-338-351
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Naphthol-AS-MX-phosphate is a chemical compound that belongs to the group of 2-naphthol derivatives.

properties

Naphtol AS-MX-Phosphate - like the phosphoric acid esters of other Naphthol-AS derivatives - is used in biochemistry as a chromogenic substrate for immunostaining with an alkaline phosphatase . After enzymatic hydrolysis of the phosphoric acid ester, a red azo dye is formed in the presence of the fast red salt TR . This method is used, for example, in Western blotting and in immunohistochemistry .

Related links

Analogues of naphthol-AS-MX-phosphate are for example:

Individual evidence

  1. a b c Data sheet Naphthol AS-MX phosphate from Sigma-Aldrich , accessed on January 2, 2015 ( PDF ).
  2. Kim S Suvarna: Bancroft's Theory and Practice of Histological Techniques. Elsevier Health Sciences, 2012, ISBN 978-0-702-05032-9 , p. 421.
  3. James W. Goding: Monoclonal Antibodies. Academic Press, 1996, ISBN 978-0-080-53695-8 , p. 418.
  4. Important naphthol AS derivatives in: Willy Herbst: Industrielle Organic Pigments. John Wiley & Sons, 2009, ISBN 978-3-527-62496-6 , p. 201 ( limited preview in Google book search).
  5. External identifiers of or database links to 6-bromo-3- (o-hydroxyphenylaminocarbonyl) -2-naphthyl phosphate: CAS number: 1919-91-1, EC number: 217-645-0, ECHA info card: 100.016.042 , PubChem : 74715 , ChemSpider : 67288 , Wikidata : Q81989818 .