para- nitrophenyl phosphate

Structural formula
General
Surname	para- nitrophenyl phosphate
other names	pNPP
Molecular formula	C 6 H 6 NO 6 P
Brief description	white-yellow, needle-shaped crystals
External identifiers / databases
CAS number 330-13-2 EC number 206-353-9 ECHA InfoCard 100.005.777 PubChem 378 ChemSpider 369 DrugBank DB04214 Wikidata Q3009340
properties
Molar mass	219.09 g mol −1 (free acid) 371.15 g mol −1 (disodium salt hexahydrate)
Physical state	firmly
Melting point	155 ° C 195 ° C (disodium salt hexahydrate)
solubility	soluble in ethanol, chloroform, benzene insoluble in water
safety instructions
GHS labeling of hazardous substances danger H and P phrases H: 315-318-373 P: 280-305 + 351 + 338
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

para -nitrophenyl phosphate is an organic chemical compound from the groups of phosphoric acid esters and nitrobenzenes . It is experimentally relevant as a substrate for the phosphatase enzyme group and is not biogenic .

use

pNPP is used as a chromogen in immunostaining for detection by alkaline phosphatase in the ELISA method. These enzymes catalyze the breakdown of pNPP to yellow p -nitrophenol .

${\displaystyle \mathrm {pNPP+H_{2}O\;{\overrightarrow {_{Al.Ph.}}}\;pNP+P_{i}} }$

Individual evidence

1. ↑ ^{a b c d} David R. Lide (Ed.): CRC Handbook of Chemistry and Physics . 89th edition. (Internet version: 2009), CRC Press / Taylor and Francis, Boca Raton, FL, Physical Constants of Organic Compounds, pp. 3-394.
2. ↑ ^{a b c} Datasheet Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution at Sigma-Aldrich , accessed on July 7, 2013 ( PDF ).
3. ↑ ^{a b} 4-nitrophenyl phosphate - disodium salt - hexahydrate BioChemica on AppliChem