|Molecular formula||C 27 H 34 I 2 N 4|
dark red, crystalline powder
|External identifiers / databases|
|Molar mass||668.40 g mol −1|
220-225 ° C
soluble in water
|As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .|
Propidium iodide ( PI ) acts like ethidium bromide as a nucleic acid intercalator . The dye can penetrate the perforated cell membrane of dead cells, but not the intact membrane of living cells . This property is in flow cytometry for discrimination of cell viability used. Another flow cytometric application of PI is the measurement of the DNA content of cells. This is mainly used to analyze the cell cycle or to determine polyploidy .
- data sheet propidium iodide (PDF) from Carl Roth , accessed on June 23, 2010.
- Propidium iodide data sheet from Sigma-Aldrich , accessed on April 22, 2011 ( PDF ).
- T. Hoppe: Studies on the developmental physiology and molecular phylogenetics of selected representatives of the myxomycetes and euglenids capable of photosynthesis (organism kingdom Protoctista) , p. 32, Kassel University Press, Kassel, 2010, ISBN 3-89958-808-8 .