Tre recombinase
The Tre recombinase is an enzyme , a recombinase that is capable of specifically the proviral DNA of the HIV virus to cut out from the DNA of human cells.
The enzyme was produced by scientists from the HPI for Experimental Virology and Immunology and the MPI for Molecular Cell Biology and Genetics through directed evolution . Starting from Cre recombinase - an enzyme that cuts out a DNA structure that closely resembles the long terminal repeats (LTR) of the HI virus - the Tre recombinase was obtained through 169 mutation steps.
The recombinase was developed by Joachim Hauber , Ilona Hauber , Frank Buchholz and Indrani Sarkar . The research results were published on June 29, 2007 in the US science journal Science . So far, the enzyme has only been used in cell culture from primary cells infected with HIV, not on whole organisms; it also takes about three months to completely remove the viral DNA in all cells. Whether and when a therapy to remove all HI viruses from human cells with the help of Tre recombinase and thus an HIV infection can be developed cannot yet be foreseen.
swell
- ↑ hpi-hamburg.de: Throwing out HIV: Foundation stone for novel therapy ( Memento of the original from December 15, 2014 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice.
- ↑ Sarkar, I. et al. (2007): HIV-1 proviral DNA excision using an evolved recombinase. In: Science. Vol. 316, pp. 1912-1915. PMID 17600219 doi: 10.1126 / science.1141453
- ↑ Technology Review: HI virus: "Tre" attacks
literature
- Frank Buchholz, Joachim Hauber: Tailor-made recombinase - a new glimmer of hope for HIV eradication. In: Retrovirus Bulletin. Vol. 2007, No. 3, pp. 9-11.