X-Gluc
Structural formula | ||||||||||||||||
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General | ||||||||||||||||
Surname | X-Gluc | |||||||||||||||
other names |
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Molecular formula | C 14 H 13 BrClNO 7 | |||||||||||||||
Brief description |
whitish powder |
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properties | ||||||||||||||||
Molar mass | 422.61 g mol −1 | |||||||||||||||
Physical state |
firmly |
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solubility |
slightly soluble in water |
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safety instructions | ||||||||||||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
X-Gluc stands for the cyclohexylammonium salt of 5-bromo-4-chloro-3-indolyl-β- D -glucuronic acid. X-Gluc is a glycoside and artificial substrate for the enzyme β-glucuronidase (GUS).
properties
The enzyme β-glucuronidase hydrolyzes X-Gluc to glucuronic acid and 5-bromo-4- chloroindoxyl . The 5-bromo-4-chloro-indoxyl is oxidized by the oxygen in the air to the deep blue dye 5,5'-dibromo-4,4'-dichloro- indigo .
use
The X-Gluc measurement is a simple optical method for checking the β-glucuronidase activity in living cells .
This method with Gus as the reporter gene can be used, for example, in plant transformations to test the transformation efficiency and, in contrast to the MUG assay, is a non-invasive method for GUS detection.
In addition, by combining the β-glucuronidase gene with the promoter of any gene, its expression can be examined in a tissue-specific manner. In contrast to the MUG assay, in which the expression can be measured quantitatively via the activity of the enzyme , the expression of a gene is qualitatively measured indirectly via the activity of the promoter.