XbaI
| Endonuclease XbaI | ||
|---|---|---|
| Mass / length primary structure | 24,700 da | |
| Identifier | ||
| Gene name (s) | XbaI (Rebase) | |
| Enzyme classification | ||
| EC, category | 3.1.21.4 , restriction enzyme | |
| Response type | hydrolysis | |
| Substrate | DNA | |
| Products | Double stranded DNA fragments with terminal 5 'phosphate groups | |
| Occurrence | ||
| Parent taxon | Xanthomonas sp. | |
XbaI is a restriction enzyme from the bacterium Xanthomonas badrii ( ATCC 11672).
properties
XbaI is an endonuclease (type II, subtype P) that cuts DNA at a palindromic DNA recognition sequence . In the presence of dimethyl sulfoxide (DMSO) or high concentrations of glycerol , the affinity of XbaI for the recognition sequence decreases and the DNA is cut unspecifically ( star activity ). The staggered cut of the DNA by XbaI results in a sticky end with a 4-base overhang and a phosphate group on each of the 5 'ends of the double-stranded DNA products. XbaI is inhibited by DAM methylation , but not by DCM or CpG methylation. After restriction of DNA in vitro , XbaI can be denatured and thus inactivated by heating at 65 ° C. for 20 minutes .
| Recognition sequence | Restriction cut |
|---|---|
5'-TCTAGA-3' 3'-AGATCT-5' |
5'-T CTAGA-3' 3'-AGATC T-5' |
Applications
XbaI is used for restriction digests in the context of cloning or restriction analyzes. Restriction analyzes with XbaI are used in human DNA to determine, among other things, DNA polymorphisms of the estrogen receptor α and ApoB .
Individual evidence
- ↑ Keith Wilson: Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press, 2010, ISBN 978-0-521-51635-8 , p. 218.
- ↑ H. Weng, C. Zhang, YY Hu, RX Yuan, HX Zuo, JZ Yan, YM Niu: Association between Estrogen Receptor-α Gene XbaI and PvuII Polymorphisms and Periodontitis Susceptibility: A Meta-Analysis. In: Disease markers. Volume 2015, 2015, p. 741972, doi : 10.1155 / 2015/741972 , PMID 26688601 , PMC 4672125 (free full text).
- ^ W. Gu, M. Zhang, S. Wen: Association between the APOB XbaI and EcoRI polymorphisms and lipids in Chinese: a meta-analysis. In: Lipids in health and disease. Volume 14, October 2015, p. 123, doi : 10.1186 / s12944-015-0125-z , PMID 26446158 , PMC 4596460 (free full text).