Calf intestinal phosphatase
| Calf intestinal phosphatase | ||
|---|---|---|
| Mass / length primary structure | 533 amino acids , 57,100 Da | |
| Secondary to quaternary structure | Homodimer | |
| Cofactor | Magnesium, zinc | |
| Identifier | ||
| Gene name (s) | IAP | |
| External IDs |
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| Enzyme classification | ||
| EC, category | 3.1.3.1 , phosphatase | |
| Response type | Hydrolysis of phosphate groups (phosphoric acid esters) | |
| Substrate | Phosphoric acid monoester + H 2 O | |
| Products | Phosphate + alcohol | |
| Occurrence | ||
| Homology family | Alkaline phosphatase | |
| Parent taxon | Bos taurus | |
In the Calf Intestinal Phosphatase ( CIP ) is an alkaline phosphatase , an in molecular biology in use enzyme which comprises the hydrolysis of free phosphate groups of biological macromolecules catalyzed . According to its name, it was first isolated from the intestinal contents of calves. Calf Intestine Phosphatase dephosphorylates mainly DNA , RNA and proteins and prefers a slightly alkaline environment as the reaction medium.
Applications
In biochemistry and molecular biology, it is particularly during cloning of plasmid DNA to prevent self-ligation after a cut with restriction enzymes employed. As an alternative, the shrimp alkaline phosphatase , which can be denatured by heating, and the Antarctic phosphatase are used.
literature
- Sambrook, J .; Maniatis, T .; Russel, DW: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press; 3rd edition (2001) ISBN 0-87969-577-3