Guanidinium thiocyanate

from Wikipedia, the free encyclopedia
Structural formula
Structure of guanidinium thiocyanate
General
Surname Guanidinium thiocyanate
other names
  • Guanidine thiocyanate
  • Guanidinium rhodanide
Molecular formula C 2 H 6 N 4 S
Brief description

white, odorless solid

External identifiers / databases
CAS number 593-84-0
EC number 209-812-1
ECHA InfoCard 100.008.922
PubChem 65046
ChemSpider 58557
Wikidata Q904487
properties
Molar mass 118.16 g mol −1
Physical state

firmly

density

1.29 g cm −3

Melting point

118 ° C

boiling point

Decomposition: from 115 ° C

solubility

very good in water (1420 g l −1 at 20 ° C)

safety instructions
GHS labeling of hazardous substances
07 - Warning

Caution

H and P phrases H: 302-312-332-412
EUH: 032
P: 273-302 + 352
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Guanidinium thiocyanate , also known as guanidine thiocyanate , is the thiocyanate salt of guanidine .

use

As a chaotrope it is used to denature proteins.

It is used in the inactivation of viruses . Guanidinium thiocyanate is also used to lyse cells and virions and for DNA and RNA isolation . A widely used method is guanidinium thiocyanate-phenol-chloroform extraction .

Individual evidence

  1. a b c d e f g guanidinium thiocyanate data sheet (PDF) from Merck , accessed on April 3, 2011.
  2. Chomczynski, P. & Sacchi, N. (1987): Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. ( Memento from February 15, 2010 in the Internet Archive ) In: Anal. Biochem. 162 (1): 156-159. PMID 2440339
  3. Chomczynski, P. & Sacchi, N. (2006): The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. (PDF; 126 kB) In: Nat. Protoc. 1 (2): 581-585. PMID 17406285
  4. Classic Protocols (2006): Purification of RNA from cells and tissues by acid phenol-guanidinium thiocyanate-chloroform extraction. (PDF; 67 kB) In: Nat. Methods. 3 (2): 149.