Amido black 10 B

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Structural formula
Structural formula of Amido Black 10 B
General
Surname Amido black 10 B
other names
  • Disodium 4-amino-5-hydroxy-3 - [(4-nitrophenyl) diazenyl] -6- (phenyldiazenyl) -2,7-naphthalene disulfonate ( IUPAC )
  • CI Acid Black 1
  • CI 20470
  • Naphthol black 6B
  • Naphthol blue black
Molecular formula C 22 H 14 N 6 Na 2 O 9 S 2
Brief description

dark brown solid

External identifiers / databases
CAS number 1064-48-8
EC number 213-903-1
ECHA InfoCard 100.012.640
PubChem 135442942
ChemSpider 21171787
Wikidata Q470900
properties
Molar mass 616.49 g mol −1
Physical state

firmly

Melting point

195 ° C

solubility

10 g l −1 (25 ° C)

safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Amidoschwarz 10 B is a bisazo dye from the application group of acid dyes . It is used in biochemical research.

presentation

Amido black 10 B is synthesized by a double azo coupling to H-acid as the coupling component. First, at a low pH, diazotized 4-nitroaniline is reacted with H-acid to form a red monoazo dye ( acidic coupling ). Coupling of the monoazo dye with diazotized aniline at high pH gives the end product ( alkaline coupling ).

use

Amido Black 10 B is used in forensic examinations to find blood on possible fingerprints . The blood proteins are colored blue-black. In a variant of Van Gieson staining , it is used with picric acid to stain collagen and reticulum . The dye has other uses in electrophoresis .

The dyeing of wool with Amido Black 10 B results in a blue-black shade with very good light fastness properties , but moderate wet fastness properties. The dye may be used to manufacture cosmetic products that only come into contact with the skin for a short time.

Individual evidence

  1. ↑ a b c data sheet Amidoschwarz 10 B (PDF) from Carl Roth , accessed on June 30, 2018.
  2. ↑ a b Data sheet Naphthol Blue Black from Sigma-Aldrich , accessed on June 30, 2018 ( PDF ).
  3. ↑ Biozym catalog, page 80 ( Memento from July 19, 2009 in the Internet Archive )
  4. ↑ F. Becker: The staining method of papierelektrophoretisch unwoven serum protein with Amido Black 10B . In: Fresenius' Journal of Analytical Chemistry , 1966 , 220, 310.
  5. ↑ Entry on naphthol black 6B. In: Römpp Online . Georg Thieme Verlag, accessed on November 13, 2019.