Brilliant sulfaflavin
Structural formula | ||||||||||||||||
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Brilliant sulfaflavin (R = CH 3 ) | ||||||||||||||||
General | ||||||||||||||||
Surname | Brilliant sulfaflavin | |||||||||||||||
other names |
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Molecular formula | C 19 H 14 N 2 O 5 S | |||||||||||||||
Brief description |
orange powder |
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External identifiers / databases | ||||||||||||||||
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properties | ||||||||||||||||
Molar mass | 382.39 g · mol -1 | |||||||||||||||
Physical state |
firmly |
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solubility |
soluble in water and ethanol |
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safety instructions | ||||||||||||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
Brillantsulfaflavin is a fluorescent dye from the naphthalimide family . Other names are Acid Yellow 7 , Brilliant Sulfoflavin FFA or Brilliant Acid Yellow 8G .
Upon excitation with violet light, for example the 439 nm mercury line, brilliant sulfaflavin emits yellow light.
use
Brilliant sulfaflavin can be used as a dye for proteins in the course of protein characterization, for example as brilliant sulfaflavin staining , which makes proteins appear yellow in the fluorescence microscope . Protein fluorescence is also used in forensics to detect blood . With blue illumination around 450 nm, traces of blood fluoresce yellow-orange. It is specifically used in finding fingerprints and footprints in blood. In histology , brilliant sulfaflavin was used for fluorescent staining of cell nuclei .
Brillantsulfaflavin is used as a dye laser with emission wavelengths between 508 nm and 573 nm in cyclooctatetraene and 522 to 618 nm in methanol . In contrast to many other laser dyes, atmospheric oxygen does not reduce the lifetime of the triplet state .
Analogues
An analogue of brilliant sulfaflavin, Brilliant Sulfoflavin FF (synonym CI 56205) has one less methyl group in the ortho position to the nitrogen (R = H in the picture) and is widely used as an alternative. The two variants are very similar chemically and physically. Sulfaflavin is also structurally related to the Lucifer fluorescent dyes.
Individual evidence
- ↑ a b c MSDS from the manufacturer MPBio , accessed on December 27, 2019.
- ↑ Data sheet Brilliant Sulfaflavine from Sigma-Aldrich , accessed on November 3, 2014 ( PDF ).
- ↑ Brilliant Sulfaflavin data sheet from Santa Cruz Biotechnology, accessed December 3, 2014.
- ↑ a b c F. WD Rost: Fluorescence Microscopy. Cambridge University Press, 1992, ISBN 978-0-521-41088-5 , pp. 378ff ( limited preview in Google book search).
- ↑ DA Freeman, HA Crissman: Evaluation of six fluorescent protein stains for use in flow microfluorometry. In: Stain technology. Volume 50, Number 4, July 1975, pp. 279-284, ISSN 0038-9153 . PMID 52920 .
- ↑ Rastogi Publications: Laboratory Manual on Biotechnology. Rastogi Publications, 2008, ISBN 978-81-7133-918-1 , p. 62ff ( limited preview in Google book search).
- ^ Robert C Shaler: Crime Scene Forensics. CRC Press, 2011, ISBN 978-1-4398-5995-7 , p. 248ff ( limited preview in Google book search).
- ↑ A detailed description can be found under: Acid Yellow 7. BVDA, accessed on December 9, 2014.
- ^ V. Sears, C. Butcher, L. Fitzgerald: Enhancement of fingerprints in blood part: reactive techniques, Acid Yellow 7, and process sequences . In: Journal of Forensic Identification . tape 55 , no. 6 , 2005, p. 741-763 .
- ↑ SK Curtis, RR Cowden: Microfluorometric estimates of proteins associated with murine hepatocyte and thymocyte nuclei, residual structures, and nuclear matrix derivatives. In: Histochemistry. Volume 82, Number 4, 1985, pp. 331-339, ISSN 0301-5564 . PMID 4008276 .
- ^ Marvin J. Weber: Handbook of Laser Wavelengths. CRC Press, 1998, ISBN 978-1-4200-5015-8 , p. 249ff ( limited preview in Google book search).
- ^ HS Freeman: Colorants for Non-Textile Applications. Elsevier, 2000, ISBN 978-0-08-052938-7 , p. 324ff ( limited preview in the Google book search).
- ^ J. Marling, D. Gregg, S. Thomas: Effect of oxygen on flashlamp-pumped organic-dye lasers . In: IEEE Journal of Quantum Electronics . tape 6 , no. 9 , September 1970, p. 570-572 , doi : 10.1109 / JQE.1970.1076525 .
- ↑ External identifiers or database links for Brilliant Sulfoflavin FF : CAS number: 10166-30-0, PubChem : 75447 , ChemSpider : 67977 , Wikidata : Q18616869 .