Ethylene glycol bis (aminoethyl ether) - N , N , N ′, N ′ -tetraacetic acid

Structural formula
General
Surname	Ethylene glycol bis (aminoethyl ether) - ; N , N , N ′, N ′ -tetraacetic acid
other names	EGTA; Ethylene bis (oxyethylene nitrilo) tetraacetic acid; 1,2-bis (2-aminoethoxy) ethane- N , N , N ′, N ′ -tetraacetic acid; Complexone VI; Titriplex VI;
Molecular formula	C 14 H 24 N 2 O 10
Brief description	White dust
External identifiers / databases
EC number	200-651-2
ECHA InfoCard	100,000,592
PubChem	6207
ChemSpider	5972
Wikidata	Q408437
properties
Molar mass	380.35 g mol −1
Physical state	firmly
Melting point	241 ° C (decomposition)
safety instructions
GHS labeling of hazardous substances	no GHS pictograms
	no GHS pictograms
H and P phrases	H: no H-phrases
	P: no P-phrases
Toxicological data	3587 mg kg −1 ( LD 50 , rat , oral )
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Ethylene glycol bis (aminoethyl ether) - N , N , N ′, N ′ -tetraacetic acid , abbreviated to EGTA, is a polyamino-carboxylic acid that is used as a chelator in chemistry and the life sciences, like the more frequently used EDTA ; but with a much higher affinity for calcium than for magnesium ions. EGTA is therefore useful for preparing buffer solutions that simulate the environment in the plasma of living cells.

The pK _s value for the binding of calcium ions from tetrabasischem EGTA is 11.0, but the protonated forms do not contribute significantly to the binding. At pH 7, the apparent pK drops _s value therefore to 6.91.

EGTA was used experimentally for the treatment of animals with cerium poisoning and for the isolation of thorium from the mineral monazite. EGTA is also part of elution buffers for Tandem Affinity Purification (TAP). Recombinant fusion proteins are bound to calmodulin spheres and released by added EGTA.

In dentistry, EGTA is used to remove deposits in the tooth root canal.

Individual evidence

↑ ^a ^b ^c ^d ^e data sheet Ethylene glycol-bis (2-aminoethylether) -N, N, N ′, N′-tetraacetic acid from Sigma-Aldrich , accessed on May 19, 2017 ( PDF ).
↑ Glenna CL Bett, Randall L. Rasmusson: Computer Models of Ion Channels . In: Candido Cabo, David S. Rosenbaum (Eds.): Quantitative cardiac electrophysiology . Marcel Dekker, 2002, ISBN 0-8247-0774-5 , p. 1–60, especially 48 ( limited preview in Google Book search).

[Sigma-1] ↑ ^a ^b ^c ^d ^e data sheet Ethylene glycol-bis (2-aminoethylether) -N, N, N ′, N′-tetraacetic acid from Sigma-Aldrich , accessed on May 19, 2017 ( PDF ).

[2] Glenna CL Bett, Randall L. Rasmusson: Computer Models of Ion Channels . In: Candido Cabo, David S. Rosenbaum (Eds.): Quantitative cardiac electrophysiology . Marcel Dekker, 2002, ISBN 0-8247-0774-5 , p. 1–60, especially 48 ( limited preview in Google Book search).