Ludwig Wilkens

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Ludwig Wilkens (born August 2, 1963 in Friesoythe, Lower Saxony ) is a German specialist in pathology and molecular pathology . He is chief physician at the Institute for Pathology at the Hannover Region Clinic .

Life

Wilkens grew up in Sedelsberg ( Saterland municipality ). After completing his military service, he studied human medicine from 1984 to 1990 and completed his training as a specialist at the Institute for Pathology and at the Institute for Cell and Molecular Pathology at the Hannover Medical School (MHH) from 1990 to 2001 . After completing his habilitation in pathological anatomy and pathology, he was senior physician and senior physician at the Institute for Pathology and at the Institute for Cell and Molecular Pathology at MHH until 2006. In 2006 Wilkens moved to the Institute for Pathology at the University of Bern as a senior physician . Wilkens has been chief physician at the Institute for Pathology at the Hannover Region Clinic since 2009.

Scientific contribution

Wilkens received his basic scientific training from Gyula Kovacs. The focus of research was the cytogenetic analysis of kidney cell tumors . Using RFLP , Wilkens analyzed cytogenetic changes on chromosome 3 in renal cell carcinomas and did his doctorate on this. After taking up his position as an assistant in pathology, Wilkens expanded his knowledge of cytogenetics and dealt with genetic changes in hematological neoplasms. He also worked on the genetics of solid tumors. He continued this work under Brigitte Schlegelberger . Wilkens showed particular interest in the analysis of the chromosomal instability of solid tumors, especially hepatocellular carcinomas (HCC) . For this purpose, HCC was systematically examined with regard to recurring chromosomal aberrations using fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) . It became clear that a distinction could be made between hepatocellular carcinomas and adenomas by means of distinct aberrations on chromosomes 1 and 8 . In addition, the genetic changes in the HCC group were compared with special consideration of the histomorphological differentiation. Overall, the dedifferentiation of HCC leads to increasing cytogenetic instability, possibly also as a consequence of telomere shortening and the occurrence of specific chromosome aberrations. Additional correlations in this direction were found in additional analyzes of gene expression . The genesis of HCC, mainly viral infections with hepatitis B and C viruses, was taken into account in further work . These processes could be confirmed in cell lines that show a basic type of chromosomal changes that are preserved over numerous passages. Ultimately, there is a tight network of the triggering agent, typical chromosomal changes, the histology and the expression of gene groups.

Memberships in scientific associations

  • German Society for Pathology
  • Professional Association of German Pathologists (State Chairman Lower Saxony)
  • International Academy of Pathology
  • International Society for Cytology
  • Swiss Society for Pathology

Publications

Research Gate publication list

Web links

Ludwig Wilkens Klinikum Hannover Institute for Pathology

Individual evidence

  1. ↑ a b Klinikum Region Hannover, Department of Pathology. Retrieved December 14, 2016 .
  2. ↑ L. Wilkens CV
  3. ↑ G. Kovacs, L. Wilkens, T. Papp: Nondisjunction reduplication of chromosome 3 is not a common mechanism in the development of human renal cell tumors. In: Cytogenetics and cell genetics. Volume 48, Number 4, 1988, pp. 242-243, PMID 3248381 .
  4. ↑ G. Kovacs, L. Wilkens, T. Papp, W. de Riese: Differentiation between papillary and nonpapillary renal cell carcinomas by DNA analysis. In: Journal of the National Cancer Institute. Volume 81, Number 7, April 1989, pp. 527-530, PMID 2921777 .
  5. ↑ G. Kovacs, C. Welter, L. Wilkens, N. Blin, W. Deriese: Renal oncocytoma. A phenotypic and genotypic entity of renal parenchymal tumors. In: The American Journal of Pathology. Volume 134, Number 5, May 1989, pp. 967-971, PMID 2719081 , PMC 1879884 (free full text).
  6. ↑ L. Wilkens, P. Komminoth, A. Nasarek, R. von Wasielewski, M. Werner: Rapid detection of karyotype changes in interphase bone marrow cells by oligonucleotide primed in situ hybridization (PRINS). In: The Journal of pathology. Volume 181, Number 4, April 1997, pp. 368-373, doi : 10.1002 / (SICI) 1096-9896 (199704) 181: 4 <368 :: AID-PATH785> 3.0.CO; 2-N , PMID 9196432 .
  7. ↑ L. Wilkens, D. Burkhardt, J. Tchinda, G. Büsche, M. Werner, M. Nolte, A. Ganser, A. Georgii: Cytogenetic aberrations in myelodysplastic syndrome detected by comparative genomic hybridization and fluorescence in situ hybridization. In: Diagnostic molecular pathology: the American journal of surgical pathology, part B. Volume 8, number 1, March 1999, pp. 47-53, PMID 10408793 .
  8. ↑ L. Wilkens, J. Tchinda, D. Burkhardt, M. Nolte, M. Werner, A. Georgii: Analysis of hematologic diseases using conventional karyotyping, fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). In: Human pathology. Volume 29, Number 8, August 1998, pp. 833-839, PMID 9712425 .
  9. ↑ L. Wilkens, M. Bredt, P. Flemming, M. Mengel, T. Becker, J. Klempnauer, H. Kreipe: Comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) in the diagnosis of hepatocellular carcinoma. In: Journal of hepato-biliary-pancreatic surgery. Volume 9, Number 3, 2002, pp. 304-311, doi : 10.1007 / s005340200034 , PMID 12353141 .
  10. ↑ a b c L. Wilkens, M. Bredt, A. Flemming, M. Mengel, J. Klempnauer, H. Kreipe, P. Flemming: Detection of chromosomal aberrations in well-differentiated hepatocellular carcinoma by bright-field in situ hybridization. In: Modern Pathology . Volume 15, number 4, April 2002, pp. 470-475, doi : 10.1038 / modpathol.3880548 , PMID 11950923 .
  11. ↑ a b L. Wilkens, M. Bredt, P. Flemming, S. Kubicka, J. Klempnauer, H. Kreipe: Cytogenetic aberrations in primary and recurrent fibrolamellar hepatocellular carcinoma detected by comparative genomic hybridization. In: American journal of clinical pathology. Volume 114, Number 6, December 2000, pp. 867-874, doi : 10.1309 / BMTT-JBPD-D13H-1UVD , PMID 11338475 .
  12. ↑ a b L. Wilkens, M. Bredt, P. Flemming, Y. Schwarze, T. Becker, M. Mengel, R. von Wasielewski, J. Klempnauer, H. Kreipe: Diagnostic impact of fluorescence in situ hybridization in the differentiation of hepatocellular adenoma and well-differentiated hepatocellular carcinoma. In: The Journal of Molecular Diagnostics: JMD. Volume 3, number 2, May 2001, pp. 68-73, doi : 10.1016 / S1525-1578 (10) 60654-X , PMID 11333302 , PMC 1907351 (free full text).
  13. ↑ a b L. Wilkens, M. Bredt, P. Flemming, T. Becker, J. Klempnauer, HH Kreipe: Differentiation of liver cell adenomas from well-differentiated hepatocellular carcinomas by comparative genomic hybridization. In: The Journal of pathology. Volume 193, Number 4, April 2001, pp. 476-482, doi : 10.1002 / path.825 , PMID 11276006 .
  14. ↑ L. Wilkens, P. Flemming, M. Gebel, J. Bleck, C. Terkamp, ​​L. Wingen, H. Kreipe, B. Schlegelberger: Induction of aneuploidy by increasing chromosomal instability during dedifferentiation of hepatocellular carcinoma. In: Proceedings of the National Academy of Sciences . Volume 101, number 5, February 2004, pp. 1309-1314, doi : 10.1073 / pnas.0305817101 , PMID 14745031 , PMC 337049 (free full text).
  15. ↑ RR Plentz, B. Schlegelberger P. Flemming, M. Gebel, H. Kreipe, MP Manns, KL Rudolph L. Wilkens: telomere shortening correlates with Increasing aneuploidy of chromosome 8 in human hepatocellular carcinoma. In: Hepatology. Volume 42, Number 3, September 2005, pp. 522-526, doi : 10.1002 / hep.20847 , PMID 16116624 .
  16. ↑ S. Hertz, T. Rothämel, B. Skawran, C. Giere, D. Steinemann, P. Flemming, T. Becker, J. Flik, ​​B. Wiese, B. Soudah, H. Kreipe, B. Schlegelberger, L Wilkens: Losses of chromosome arms 4q, 8p, 13q and gain of 8q are correlated with increasing chromosomal instability in hepatocellular carcinoma. In: Pathobiology. Volume 75, number 5, 2008, pp. 312-322, doi : 10.1159 / 000151712 , PMID 18931534 .
  17. ↑ B. Skawran, D. Steinemann, T. Becker, R. Buurman, J. Flik, ​​B. Wiese, P. Flemming, H. Kreipe, B. Schlegelberger, L. Wilkens: Loss of 13q is associated with genes involved in cell cycle and proliferation in dedifferentiated hepatocellular carcinoma. In: Modern Pathology. Volume 21, number 12, December 2008, pp. 1479-1489, doi : 10.1038 / modpathol.2008.147 , PMID 18820673 .
  18. Jump up ↑ L. Wilkens, R. Jaggi, C. Hammer, D. Inderbitzin, O. Giger, N. von Neuhoff: The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. In: Virchow's archive . Volume 458, Number 6, June 2011, pp. 697-708, doi : 10.1007 / s00428-011-1070-5 , PMID 21484430 .
  19. ↑ B. Skawran, D. Steinemann, A. Weigmann, P. Flemming, T. Becker, J. Flik, ​​H. Kreipe, B. Schlegelberger, L. Wilkens: Gene expression profiling in hepatocellular carcinoma: upregulation of genes in amplified chromosome regions. In: Modern Pathology. Volume 21, number 5, May 2008, pp. 505-516, doi : 10.1038 / modpathol.3800998 , PMID 18277965 .
  20. ↑ L. Tan, T. Meier, M. Kuhlmann, F. Xie, C. Baier, Z. Zhu, WM Cong, L. Wilkens: Distinct set of chromosomal aberrations in childhood hepatocellular carcinoma is correlated to hepatitis B virus infection. In: Cancer Genetics. Volume 209, number 3, March 2016, pp. 87-96, doi : 10.1016 / j.cancergen.2015.12.010 , PMID 26837720 .
  21. ↑ L. Wilkens, C. Hammer, S. Glombitza, DE Müller: Hepatocellular and cholangiolar carcinoma-derived cell lines reveal distinct sets of chromosomal imbalances. In: Pathobiology. Volume 79, number 3, 2012, pp. 115-126, doi : 10.1159 / 000334100 , PMID 22261732 .
personal data
SURNAME Wilkens, Ludwig
BRIEF DESCRIPTION German pathologist
DATE OF BIRTH 2nd August 1963
PLACE OF BIRTH Friesoythe, Lower Saxony