2- ( N -morpholino) ethanesulfonic acid

Structural formula
General
Surname	2- ( N -morpholino) ethanesulfonic acid
other names	MES
Molecular formula	C 6 H 13 NO 4 S
Brief description	colorless solid
External identifiers / databases
CAS number 4432-31-9 145224-94-8 (monohydrate) EC number 224-632-3 ECHA InfoCard 100.022.394 PubChem 78165 ChemSpider 70541 DrugBank DB03814 Wikidata Q209294
properties
Molar mass	195.20 g mol −1
Physical state	firmly
Melting point	316 ° C
solubility	soluble in cold water
safety instructions
GHS labeling of hazardous substances Caution H and P phrases H: 315-319-335 P: 261-305 + 351 + 338
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

2- ( N -Morpholino) ethanesulfonic acid , also abbreviated as MES , is a synthetically produced buffer substance from the group of Good buffers that is frequently used in the life sciences . The pK _s value of MES is 6.1 (25 ° C), the buffer range is pH 5.8-6.5. MES is structurally closely related to MOPS , which is the propanesulfonic acid derivative of MES.

MES is not metabolized by bacteria and eukaryotic cells, which is why it is often used for the production of weakly acidic buffered cultivation media, for example for plant tissue at pH 5.8. To produce sterile solutions, MES-containing solutions are sterile-filtered because the substance is destroyed by autoclaving. Another possibility is to prepare the solution under sterile conditions.

See also

• HEPES
• HEPPS
• TRIS
• PUG

Individual evidence

1. ↑ ^{a b c d} Entry on 2-morpholinoethanesulfonic acid. In: Römpp Online . Georg Thieme Verlag, accessed on April 20, 2014.
2. ↑ ^{a b} MES monohydrate data sheet from Sigma-Aldrich , accessed on March 19, 2011 ( PDF ).
3. ↑ Data sheet 2- (N-Morpholino) ethanesulfonic acid from Sigma-Aldrich , accessed on January 15, 2011 ( PDF ).