Fructose-1,6-bisphosphatase

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Fructose-1,6-bisphosphatase
Fructose-1,6-bisphosphatase
Ribbon model of the tetramer according to PDB  1FTA

Existing structural data : 1fta , 2fhy , 2fie , 2fix , 2jjk , 2vt5

Mass / length primary structure 337 amino acids
Secondary to quaternary structure Homotetramer
Cofactor zinc
Identifier
Gene name (s) FBP1 , FBP2
External IDs
Enzyme classification
EC, category 3.1.3.11 ,  phosphatase
Response type Hydrolysis of a phosphoric acid ester bond
Substrate D-fructose-1,6-bisphosphate + H 2 O
Products D-fructose-6-phosphate + phosphate
Occurrence
Homology family FBPase
Parent taxon Creature

Fructose-1,6-bisphosphatase (abbr .: FBPase ) is the name of the enzyme that breaks down fructose-1,6-bisphosphate into fructose-6-phosphate . It is part of several metabolic pathways and is found in most organisms. In humans there are two isoforms ( genes FBP1 and FBP2 ) that are localized in the liver and muscles . Mutations in the FBP1 gene can lead to a deficiency in FBPase.

FBPase-1 is mainly localized in the liver and kidneys, as well as in the ileum , in leukocytes and in the brain. Although it is relatively unstable in the urine, its presence there can be used as a marker for proximal tubular kidney damage .

Catalyzed reaction

F16BP+ H 2 O   + P i  F6P

Fructose-1,6-bisphosphate is hydrolyzed to fructose-6-phosphate and phosphate. This reaction is part of gluconeogenesis and the pentose phosphate pathway , as well as the Calvin cycle in plants and the dihydroxyacetone cycle in methylotrophic organisms. The latter can build up cell material from C 1 compounds such as methanol , methylamine or methyl ether compounds.

regulation

While the activity of FBPase is restricted by the presence of fructose-2,6-bisphosphate and AMP , the transcription factors USF1 , Sp1 and NF-κB bind to the promoter of the FBP1 gene .

Individual evidence

  1. ↑ UniProt P09467
  2. ↑ Kepka A, Szajda SD, Zwierz K: [Fructose-1,6-bisphosphatase - marker of damage to proximal renal tubules] . In: Pol. Mercury. Lekarski . 24, No. 140, February 2008, pp. 125-30. PMID 18634268 .
  3. ↑ Kepka A, Dariusz Szajda S, Stypułkowska A, et al : Urinary fructose-1,6-bisphosphatase activity as a marker of the damage to the renal proximal tubules in children with idiopathic nephrotic syndrome . In: Clin. Chem. Lab. Med. . 46, No. 6, 2008, pp. 831-5. doi : 10.1515 / CCLM.2008.171 . PMID 18601606 .
  4. ↑ Wolfgang Babel: Metabolic Cycles - biochemically necessary and useful. In: BIOspectrum | 07.09 | 15th year; PDF (free full text)
  5. ↑ Herzog B, Waltner-Law M, Scott DK, Eschrich K, Granner DK: Characterization of the human liver fructose-1,6-bisphosphatase gene promoter . In: Biochem. J. . 351 Pt 2, October 2000, pp. 385-92. PMID 11023824 . PMC 1221374 (free full text).

Web links

Wikibooks: Biochemistry and Pathobiochemistry: Fructose-1,6-bisphosphatase  - learning and teaching materials