Lillie trichrome stain

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The Lillie's trichrome is a histological trichrome staining method .

properties

Most variants of the Lillie stain produce red colored keratin and muscle fibers , blue or green collagen and bone , pink cytoplasm, and brown-black cell nuclei . After fixing, the objects to be stained are immersed in iron trioxyhematein according to Hansen or iron trioxyhematein according to Hansen to stain the cell nuclei. This is followed by cytoplasmic staining with Biebricher scarlet fever in an aqueous acetic acid solution. The next solution contains tungstophosphoric acid and molybdophosphoric acid . Finally, the protein fibers are dyed with Fast Green FCF . Alternatively, a blue coloration with methylene blue , water blue or aniline blue can be used.

variants

The Masson trichrome stain uses acid fuchsin and Ponceau 2R as the red dye and light green SF as the green dye . The Masson-Goldner trichrome stain also uses orange G , but does not contain phosphotungstic acid. The Masson Trichrom & Verhoeff stain is a combination of stains that is used to stain blood vessels and to differentiate between arteries and veins , as the Verhoeff stain stains elastin black.

history

Lillie trichrome staining was developed in 1945 by Ralph Dougall Lillie (1896–1979). It is a further development of the Masson trichrome staining .

literature

  • J. Bancroft, M. Gamble: Theory and Practice of Histological Techniques. 6th edition, Churchill-Livingstone, London 2008.
  • B. Bricegirdle: A History of Microtechnique. 2nd edition, Science Heritage Ltd, Chicago 1986.
  • FL Carson: Histotechnology A Self Instructional Text. 2nd edition, ASCP Press, Chicago 1997.
  • D. Sheehan, BB Hrapchak: Theory and Practice of Histotechnology. 2nd edition, Mosby, St. Louis 1980.

Web links

  • Bryan D. Llewellyn: Differential Staining With Acid Dyes. PDF .

Individual evidence

  1. ^ A b R. D. Lillie: Studies on selective staining of collagen with acid aniline dyes. In: J. Tech. Meth. (1945), Vol. 25, pp. 1-47.
  2. ^ P. Masson: Some histological methods; trichrome stainings and their preliminary technique. In: J. Tech. Methods (1929) Vol. 12, pp. 75-90.
  3. ↑ Jacques Goldner: A modification of the masson trichrome technique for routine laboratory purposes. In: Am J Pathol. (1938), Volume 14, Issue 2, pp. 237-243. PMC 1964940 (free full text).
  4. ↑ Masson Trichrome & Verhoeff Stain ( Memento of the original from September 27, 2011 in the Internet Archive ) Info: The archive link has been inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. @1@ 2Template: Webachiv / IABot / education.vetmed.vt.edu
  5. ^ P. Masson: Some histological methods; trichrome stainings and their preliminary technique. In: J. Tech. Methods (1929) Vol. 12, pp. 75-90.
  6. ↑ Gudrun Lang: Trichrome stains or: What makes fibers blue and muscles red? In: Science & Practice. Pp. 10-15. limited preview in Google Book search.