Nitro blue tetrazolium chloride

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Structural formula
Structural formula of nitro blue tetrazolium chloride
General
Surname Nitro blue tetrazolium chloride
other names

3,3 ′ - (3,3′-Dimethoxy-4,4′-biphenylylene) -bis- [2- (4-nitro-phenyl) -5-phenyl-2 H -tetrazolium chloride]

Molecular formula C 40 H 30 Cl 2 N 10 O 6
Brief description

yellow crystals

External identifiers / databases
CAS number 298-83-9
EC number 206-067-4
ECHA InfoCard 100.005.517
PubChem 9281
ChemSpider 8924
Wikidata Q906440
properties
Molar mass 817.65 g mol −1
Physical state

firmly

Melting point

189 ° C

solubility

bad in water

safety instructions
GHS labeling of hazardous substances
07 - Warning

Caution

H and P phrases H: 302
P: no P-phrases
Toxicological data

2000 mg kg −1 ( LD 50 ,  mouse ,  oral )

As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Nitro blue tetrazolium chloride ( NBT ), a quaternary ammonium compound is a di- tetrazolium salt and redox - dye .

properties

By reducing the di-tetrazolium salt, a red mono- formazan dye is initially produced . Further reduction produces a blue di-formazan dye.

use

In biochemistry , NBT is used in the course of immunostaining in combination with 5-bromo-4-chloro-3-indoxyl phosphate ( BCIP) for the sensitive detection of the enzyme alkaline phosphatase (AP). In medicine, NBT is used to diagnose septic granulomatosis .

Individual evidence

  1. ↑ a b c d Data sheet nitro blue tetrazolium chloride (PDF) from Merck , accessed on March 15, 2010.
  2. ↑ Safety data sheet Sigma-Aldrich, accessed on April 17, 2015.
  3. ↑ Entry on nitro blue tetrazolium chloride in the ChemIDplus database of the United States National Library of Medicine (NLM) .
  4. ↑ McGadey, J. (1970): A tetrazolium method for non-specific alkaline phosphatase. In: Histochemistry. Vol. 23, pp. 180-184; PMID 5507043 ; doi: 10.1007 / BF00305851 .
  5. ↑ Knecht, DA et al. (1984): Visualization of antigenic proteins on Western blots. In: Anal. Biochem. Vol. 136, pp. 180-184; PMID 6201085 ; doi: 10.1016 / 0003-2697 (84) 90321-X .