Janus green B

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Structural formula
Structural formula of Janusgrün B
General
Surname Janus green B
other names
  • 3- (Diethylamino) -7 - {( E ) - [4- (dimethylamino) phenyl] diazenyl} -5-phenylphenazin-5-ium chloride ( IUPAC )
  • CI 11050
  • Diazine green
Molecular formula C 30 H 31 ClN 6
Brief description

black powder

External identifiers / databases
CAS number 2869-83-2
EC number 220-695-6
ECHA InfoCard 100,018,814
PubChem 76123
ChemSpider 68607
Wikidata Q418288
properties
Molar mass 511,06 g · mol -1
Physical state

firmly

solubility

very heavy in water (20 mg l −1 at 20 ° C)

safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Janusgrün B is a chemical compound from the group of azo dyes (monoazo dye) based on phenazine . It is in the form of a black powder.

presentation

Janus Green B can be prepared by diazotization of the N , N -Diethylphenosafranin and coupling to N , N -dimethylaniline can be produced.

use

Janus green B is used as a dye in microscopy and histology . It is a so-called vital pigment, which means that the stained structures such as mitochondria , leukocytes and embryonic tissue remain functional.

The fact that Janus green is suitable for staining mitochondria was discovered by Leonor Michaelis and published in 1900.

Janus green B is also used as a dye for the counterstaining in the modified Ziehl-Neelsen staining . In the presence of an oxidizer, the dye turns blue through oxidation , otherwise pink through reduction .

Individual evidence

  1. ↑ a b Datasheet Janus Green B at AlfaAesar, accessed on February 10, 2018 ( PDF )(JavaScript required) .
  2. ↑ a b Datasheet Janus Green B at Sigma-Aldrich , accessed on February 10, 2018 ( PDF ).
  3. ↑ External identifiers of or database links to 3-Amino-7- (diethylamino) -5-phenylphenazin-5-ium chloride : CAS number: 4569-86-2, EC number: 610-268-6, ECHA - InfoCard: 100.118.786 , PubChem : 2733867 , ChemSpider : 2015631 , Wikidata : Q72490509 .
  4. ^ Leonor Michaelis. 1875–1949 (PDF; 2.4 MB). A Biographical Memoir by L. Michaelis, DA MacInnes and S. Granick J., National Academy of Sciences.
  5. ^ Entry Janusgrün B (CI 11050) at Carl Roth , accessed on February 10, 2018.
  6. ↑ ANAEROBIC RESPIRATION IN YEAST ( Memento of February 24, 2006 in the Internet Archive ); Internship attempt (English, MS Word ; 24 kB).