Cell type markers

A lineage markers , and cell lineage markers ( English cell lineage markers ) or differentiation markers , is an a cell type characteristic molecule, z. B. a surface antigen , an mRNA or a protein in a cell .

properties

Cell type markers are used in biochemistry and cell biology as biomarkers to determine the cell type and cell origin . With the help of antibodies against the surface antigens, cells with certain cell type markers can be detected in the course of immune staining , e.g. B. by flow cytometry , immunohistochemistry or immunofluorescence . Individual cell types can also be enriched with antibodies against the surface antigens, e.g. B. via FACS or via MACS .

Human cell type markers

Cell type markers of humans on the cell surface are z. B. CD3 on T lymphocytes , CD14 on monocytes , CD16 and CD56 on NK cells and granulocytes , CD19 and CD20 on B lymphocytes . They are partly depending on the developmental stage of a cell type and are only in some stages expressed . Cell type markers for embryonic stem cells are e.g. B. Nanog , Oct-4 , Sox-2 , Rex-1, Dnmt3b, Lin-28 , Tdgf1, FoxD3, Tert , Utf-1, Gal, Cx43, Gdf3, Gtcm1, Terf1, Terf2, Lefty A and Lefty B, in contrast, cell type markers for mesenchymal stem cells , e.g. B. CD13 , CD29 , CD44 , CD49e , CD54 , CD71 , CD73 , CD90 , CD105 , CD106 , CD166 and HLA-ABC .

Web links

Mesenchymal Stem Cells and Lineage specific markers ( Memento from November 6, 2014 in the Internet Archive ).

Individual evidence

↑ ^a ^b ^c ^d R. Calloni, EA Cordero, JA Henriques, D. Bonatto: Reviewing and updating the major molecular markers for stem cells. In: Stem Cells and Development . Volume 22, number 9, May 2013, pp. 1455–1476, doi: 10.1089 / scd.2012.0637 , PMID 23336433 , PMC 3629778 (free full text).
↑ P. Saussoy, JL Vaerman, V. Druez, V. Deneys, N. Straetmans, G. Cornu, A. Ferrant, D. Latinne: Expression of lineage markers using real-time quantitative polymerase chain reaction (RT-qPCR) in normal and in leukemia bone marrow. In: Clinical Chemistry and Laboratory Medicine . Volume 47, number 4, 2009, ISSN 1434-6621 , pp. 419-426, doi: 10.1515 / CCLM.2009.093 , PMID 19327122
↑ KK Lin, MA Goodell: Detection of hematopoietic stem cells by flow cytometry. In: Methods in Cell Biology . Volume 103, 2011, ISSN 0091-679X , pp. 21-30, doi: 10.1016 / B978-0-12-385493-3.00002-4 , PMID 21722798 .
↑ T. Nagasawa: Micro Environmental niches in the bone marrow required for B-cell development. In: Nature Reviews Immunology . Volume 6, Number 2, February 2006, pp. 107-116, doi: 10.1038 / nri1780 , PMID 16491135 .

[PMID_23336433-1] R. Calloni, EA Cordero, JA Henriques, D. Bonatto: Reviewing and updating the major molecular markers for stem cells. In: Stem Cells and Development . Volume 22, number 9, May 2013, pp. 1455–1476, doi: 10.1089 / scd.2012.0637 , PMID 23336433 , PMC 3629778 (free full text).

[2] P. Saussoy, JL Vaerman, V. Druez, V. Deneys, N. Straetmans, G. Cornu, A. Ferrant, D. Latinne: Expression of lineage markers using real-time quantitative polymerase chain reaction (RT-qPCR) in normal and in leukemia bone marrow. In: Clinical Chemistry and Laboratory Medicine . Volume 47, number 4, 2009, ISSN 1434-6621 , pp. 419-426, doi: 10.1515 / CCLM.2009.093 , PMID 19327122

[3] KK Lin, MA Goodell: Detection of hematopoietic stem cells by flow cytometry. In: Methods in Cell Biology . Volume 103, 2011, ISSN 0091-679X , pp. 21-30, doi: 10.1016 / B978-0-12-385493-3.00002-4 , PMID 21722798 .

[4] T. Nagasawa: Micro Environmental niches in the bone marrow required for B-cell development. In: Nature Reviews Immunology . Volume 6, Number 2, February 2006, pp. 107-116, doi: 10.1038 / nri1780 , PMID 16491135 .