Cetyltrimethylammonium bromide
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| Surname | Cetyltrimethylammonium bromide | |||||||||||||||||||||
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| Molecular formula | C 19 H 42 BrN | |||||||||||||||||||||
| Brief description |
white solid with a faint odor |
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| Molar mass | 364.45 g mol −1 | |||||||||||||||||||||
| Physical state |
firmly |
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| Melting point |
248-251 ° C |
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| solubility |
heavy in water (3.1 g l −1 at 19.35 ° C) |
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| As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . | ||||||||||||||||||||||
Cetyltrimethylammonium bromide (CTAB or CTABr) is a quaternary ammonium compound with a long-chain alkyl group with 16 carbon atoms.
properties
CTAB is a cationic surfactant that is used as a detergent . Depending on the salt concentration in the solution, it forms ion pairs (salts) with nucleic acids such as DNA and RNA , which are soluble in water but insoluble in concentrated alcohol. CTAB solutions are stable for several years at room temperature. It is in the form of a white, crystalline powder.
use
CTAB is used
- as a phase transfer catalyst for polyamides , polycarbonates and polythiocarbonates .
- in biochemistry .
- in DNA extraction ( plasmid isolation). With this method, interfering polysaccharides and proteins are separated by complex formation and precipitation of the DNA with CTAB (CTAB method).
- as a cationic surfactant in chromatography .
- in CTAB-PAGE , a gel electrophoresis to determine the molar mass of proteins that show unusual migration behavior (e.g. strongly positively charged proteins or membrane protein subunits).
- in the precipitation of high molecular weight DNA, especially from plant material (genomic DNA). The tissue or cells are homogenized in buffers containing CTAB.
- as an additive in ion pair chromatography.
- as a titrant for potentiometric titration of perchlorates with ion-selective electrodes .
- as a preservative and disinfectant (C. is an effective antiseptic against bacteria and fungi.) and is contained in cosmetics and hair care products, sanitary cleaners , vaccines and softeners .
- as an active ingredient in drugs (e.g. in oral and throat therapeutics).
- as an additive in epoxy resin coatings .
- as a surfactant in capillary electrophoresis (CE) to reverse the electroosmotic flow (EOF).
- as a corrosion inhibitor for CO 2 corrosion of iron.
- As a component for culture media ( cetrimide agar ) for the detection of bacteria (including Pseudomonas aeruginosa ).
- as a stabilizer in the synthesis of inorganic nanoparticles
- for the introduction of mesopores in zeolite Y
Related links
Individual evidence
- ↑ a b c d e f Entry on cetyltrimethylammonium bromide in the GESTIS substance database of the IFA , accessed on January 10, 2017(JavaScript required) .
- ↑ JM Pollard, AJ Shi, KE Göklen: Solubility and Partitioning Behavior of Surfactants and Additives Used in Bioprocesses . In: J. Chem. Eng. Data 51 (2006) 230-236, doi : 10.1021 / je0503498 .
- ^ Joint Genome Institute : Bacterial genomic DNA isolation using CTAB
- ↑ Engelbert Buxbaum: Cationic electrophoresis and electrotransfer of membrane glycoproteins . In: Analytical Biochemistry . tape 314 , no. 1 , March 2003, p. 70-76 , doi : 10.1016 / S0003-2697 (02) 00639-5 ( elsevier.com [accessed August 19, 2020]).
- ↑ García-Martínez, J., et al., Mesostructured zeolite Y - high hydrothermal stability and superior FCC catalytic performance , Catal. Sci. Technol. , 2012, 2 , 987-994.