Myskina joined the professional circus in 1998 and reached the top 500 in the world rankings that same year . Just one year later she was in the top 100 and at the end of 2002 in the top 20. In 2003 she was among the top ten tennis players in the world. She achieved her best world ranking with second place in September 2004.
In her professional career, she won ten singles and five doubles titles on the WTA Tour . Her greatest success came on June 5, 2004 when she won the French Open , when she clearly defeated her compatriot Jelena Dementjewa 6: 2, 6: 1 in the final. Myskina was the first Russian female tennis player to win an individual title in a Grand Slam tournament . In 2004, as in the previous year, she won the Kremlin Cup title in her hometown of Moscow . There, too, she defeated Dementjewa (7: 5, 6: 0).
When she was eliminated in the first round in 2005 , she was the first player who, as defending champion of the French Open, could not win a match the next time she participated. Her mother's cancer was cited as one reason for this, also for further failures in 2005. In 2005 she reached the quarter-finals for the first time in her career at Wimbledon .
From 1998 to 2005 Myskina completed 24 games for the Russian Fed Cup team . She celebrated 18 wins, 14 of them individually.
In 2007, she was eliminated again in the first round of the French Open. On July 4, 2007, Anastassija Myskina announced that she would no longer play tournaments because of her foot injury.