Helena Ekholm made her debut in Östersund in 2005 in a relay race (15th) in the Biathlon World Cup . At the following World Cup in Hochfilzen , she contested her first individual race (41st). In spring 2006 she took part in the Mixed World Championship in Pokljuka and was sixth. After she did not get past a 16th place in her first season, the following season began quite successfully for her. At the third season World Cup in Hochfilzen, she came fourth in the individual and eighth in the sprint. Also in the season she was fourth with the Swedish team. At the Biathlon World Championships 2007 in Antholz , she continued her upward trend and also took fourth place in the sprint. As a highlight, she won the gold medal with Sweden's mixed relay. She celebrated her first World Cup victory in the last race of the 2006/07 season , a mass start race in Khanty-Mansiysk .
At the World Cup opening of the 2008/09 season , Helena Ekholm was able to achieve her second individual World Cup success and the first in the individual competition in front of her own audience in Östersund with a faultless shooting. In the pursuit of the Biathlon World Championships in 2009 , she started from fifth place and achieved the world championship title through good shooting performance, and she also won silver in the mixed relay and bronze in the mass start. After the sprint race at the World Cup in Vancouver , she won the yellow jersey of the overall World Cup leaders with her fourth win of the season. She finally crowned the season by winning the overall World Cup. Although she was tied with the German Kati Wilhelm in first place, the overall World Cup victory went to Ekholm due to the higher number of victories this season. The Swede also won the Sprint and Mass Start World Cup this season. In 2010 Ekholm took part in the Winter Olympics in Vancouver. Her best result was a tenth place in the mass start. With the relay she finished fifth. She got off to a furious start in the World Cup, winning two of the first five races of the season in addition to the first race of the season, an individual in Östersund. But she did not maintain this level for the whole season and in the end had to admit defeat to Magdalena Neuner and Simone Hauswald and came third. After the 2011/12 season, Helena Ekholm ended her career.
Helena Ekholm has been married to the Swedish biathlete David Ekholm since July 17, 2010 . She has three younger siblings, Jenny Jonsson , Malin Jonsson and Mattias Jonsson, who are all also active in biathlon.