The line was electrified in 1961 (Přerov – Petrovice u Karviné) and 1981–1985 (Břeclav – Přerov).
On January 1, 1993, the line was transferred to the newly founded České dráhy (ČD) in the course of the dissolution of Czechoslovakia . Since 2003 it has been part of the network of the state infrastructure operator Správa železniční dopravní cesty (SŽDC).
At Studénka, on August 8, 2008, the EuroCity Comenius drove into the rubble of a bridge that had recently collapsed. Eight people died in the worst railway accident in the history of the independent Czech Republic.
With the timetable change on December 9, 2012, the Břeclav – Bohumín section was switched to right-hand drive.
On July 22, 2015, a railcar of the ČD class 680 as SC 512 Bohumín - Františkovy Lázně collided with a broken down truck on a level crossing near Studénka . Two people were killed and others were injured, some seriously.
On February 25, 2019, the route operator SŽDC put the conversion of the overhead line supply from 3 kV direct voltage to 25 kV 50 Hz alternating voltage in the Nedakonice – Říkovice section out to tender. The tender was won by a consortium of Elektrizace železnic Praha and AŽD Praha, which had submitted the cheapest offer with a planned cost of 2.1 billion crowns. Work is scheduled to begin in April 2020 and completion in August 2022.
In December 2019, the route operator SŽDC publicly tendered the renewal of the approximately ten-kilometer section from Dětmarovice to the Polish state border. It is calculated with costs of 2945 million crowns, which are to be financed proportionately through European funds, loans and the state fund for transport infrastructure. Completion is scheduled for 2020.
